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莪术油对人乳腺癌MCF7细胞凋亡及Bcl2、Bax表达的影响
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【中医学生暑期实践论文】【摘要】 目的:观察中药提取物莪术油(zedoray turmeric oil)对人乳腺癌(human breast cancer)细胞系MCF7的凋亡诱导作用。方法:用不同浓度的莪术油对体外培养的MCF7细胞进行干预。分别采用MTT、细胞免疫组织化学染色、流式细胞仪检测等方法,观察其对MCF7细胞增殖的抑制和凋亡诱导作用,并对凋亡相关Bcl2与Bax蛋白的表达变化进行定性检测。结果:经不同浓度的莪术油处理后的MCF7细胞,其生长受到明显的抑制,细胞凋亡指数随药物浓度增加而明显增加;莪术油作用后,细胞中Bax蛋白表达明显高于对照组。结论:一定浓度的莪术油能抑制MCF7细胞增殖,促进其凋亡;其机制可能与调控Bcl2、Bax 表达有关。 【关键词】 乳腺癌 莪术油 MCF7细胞 Bcl2 Bax Abstract Objective:To investigate the effects of Zedoray Turmeric Oil on inducing apoptosis of MCF7 cells and to explore the possible mechanism of its action.Methods:Different concentrations of Zedoray Turmeric Oil were used in the culture system of MCF7 cells.Proliferation and apoptosis of MCF7 cells were observed by ways of MTT,immunocytochemistry,and flow cytometry quantitative analysis.The expression of Bcl2 and Bax protein was determined by qualitative analysis.Results:Proliferation of MCF7 c
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