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用庆大霉素抗性筛选法选育西罗莫司高产菌株
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【西药论文】【摘要】 用紫外线对吸水链霉菌(Streptomyces hygroscopicus)NP03进行诱变，再采用庆大霉素抗性筛选法进行处理，剂量分别为0、0.01、0.03、0.05和0.08μg/ml，共获得113株抗性突变株，其中西罗莫司产量高于出发菌株的有51株，突变株NP0374#的生产能力达到出发菌株的134%，且遗传特性稳定。
【关键词】 西罗莫司； 庆大霉素； 抗性筛选； 遗传稳定性
Screening of mutant overproducing sirolimus with
gentamicin resistance method
Zhang Li and Jia Xiaojing
(New Drug Research and Development Center, North China Pharmaceulical Co., Shijiazhuang 050015)
ABSTRACT After the spores of Streptomyces hygroscopicus NP03 being treated with UV, as a mutagen one hundred thirteen sirolimusproducing strains were obtained by gentamicin resistance screening. The treatment dose was 0, 0.01, 0.03, 0.05 and 0.08μg/mg respectively. The sirolimus yields of 51 mutants were higher than that of the original strain. Among them, the yield of #NP0374 was 134% as high as original strain′s, and the characteristic can be ste
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