|
抗耐甲氧西林金黄色葡萄球菌(MRSA)卵黄免疫球蛋白(IgY)的制备与性质研究
|
|
【康复西医学论文】黄清松，罗深秋，林元藻，曾位森【摘要】 目的 观察鸡卵黄中抗耐甲氧西林金黄色葡萄球菌(methicillinresistant staphylococcus aureus,MRSA)抗体的含量、纯度、来源及稳定性。 方法 用耐甲氧西林金黄色葡萄球菌(MRSA)作为抗原免疫Leghorn鸡；通过改良水稀释法提取卵黄中的IgY；双紫外光波长测定抗体含量,SDSPAGE电泳检测抗体纯度；Western blot免疫印迹法测定该抗体来源；ELISA检测IgY对温度、酸碱度的稳定性。结果 抗体含量13.3 mg/mL蛋黄液,抗体纯度达96%；免疫印迹证明IgY与鸡血清中的IgG具有相同的分子量和抗原性；IgY具有良好的热稳定性,对酸碱具有一定的耐受力。结论 改良水稀释法能得到高产量、高纯度的特异性IgY,而且有良好的生物学活性。
Abstract:Objective To observe the yield, purity, source, and stability of the methicillinresistant staphylococcus aureus (MRSA). Methods Taken the methicillinresistant staphylococcus aureus (MRSA) as antigen immuned Leghorn fowl, IgY was extracted with improved water dilution method. Antibody’s content was determined with double beam UV/VIS spectrophotometer, and antibody purity was detected with SDSPAGE electrophoresis. Antibody’s source was determined by Western blot. IgY’s constancy to temperature and ac
|
|
|
广告服务