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负载甘露糖化抗原的DC诱导的特异性杀伤
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【临床西医学毕业论文】作者：钟国成, 徐明, 施明, 胡美茹, 闵敏, 陈健【关键词】 树突状细胞;,糖基化抗原;,肿瘤免疫Specific cytotoxicity of CTL which was induced by DC pulsed with mannosylated antigen
[Abstract] AIM: To investigate the role of mannosylated tumor antigen in the course of antitumor response. METHODS: L2 domain of HER2/neu (ErbB2) ectodomain was expressed in E.coli, purified and mannosylated. Dendritic cells(DCs) were induced from human peripheral blood mononuclear cell by GMCSF and IL4. The maturation and functional capacity of DCs pulsed with mannosylated L2 (mL2) protein was investigated. RESULTS: L2 protein could induce DC maturation, which was accompanied by elevated expression of MHC and costimulatory molecules. The effect of mL2 protein on DC maturation was more remarkable than that of nonmannosylated L2 protein. Furthermore, DCs pulsed with mL2 could stimulate high tumor cell lysis by CTL. CONCLUSION: Our ex
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