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结核杆菌H37Ra免疫小鼠后脾淋巴细胞的杀伤活性及免疫机制的研究
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【西药学科研论文】作者：王利娴, 卢贤瑜, 李惠, 阳强【关键词】 结核杆菌H37Ra;,Ag85B蛋白;,小鼠淋巴细胞;,穿孔素;,颗粒酶BStudy on the cytotoxicity of spleen lymphocytes and immune mechanisms in mice immunized by Mycobacterium tuberculosis H37Ra
[Abstract] AIM: To explore the specific cytotoxicity of spleen lymphocytes and the immune mechanisms in mice immunized by Mycobacterium tuberculosis（MTB）H37Ra. METHODS: At the various interval (30days, 60days) after the mice were immunized by MTB H37Ra, BCG and PBS, the spleen lymphocytes of the immunized mice were used as the effector cells while the Sp2/0 cells expressing the secreted protein Ag85B were used as the target cells, and the cytotoxicity of spleen lymphocytes in the immunized mice was measured by MTT assay. Spleen lymphocytes were collected at 60 days after immunization and stimulated with PPD, and the expression of perforin, granzyme B on mRNA level was detected by RTPCR. R
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